蛋白-蛋白相互作用

Captured method

ka (1/Ms)kd (1/s)KD (M)
4.788E+062.612E-045.455E-10

Amine coupling method

ka (1/Ms)kd (1/s)KD (M)
3.539E+60.011633.287E-9

Protein-protein binding analysis using Biacore. The left figure is about binding of antigen to antibody. The right figure is about binding of a molecular chaperone GroEL to cochaperone GroES.

蛋白-化合物相互作用

Case 1: KD=126 mM


Case 2:
KD=144 nM; kd=0.13 s-1

Case 3: KD=1.97 nM; kd=0.02 s-1

Biacore binding assays developed for more than 30 enzymes with small molecules or peptides. These figures show the affinities we have tested range from mM to nM.

片段库筛选

Example: Screening plate results

Example of selected hits: KD in several hundred ?M

Fragment library screening service using Biacore T200 and 384-well plate. 350 fragments can be screened every day. This chart shows an example of screening of 3600 fragments. 41 hit were selected for further affinity characterization.